abstract：:The ability to form teratomas in vivo containing multiple somatic cell types is regarded as functional evidence of pluripotency for human pluripotent stem cells (hPSCs). Since the Teratoma assay is animal dependent, laborious, and only qualitative, the PluriTest and the hPSC ScoreCard assay have been developed as in v...

journal_title：Stem cell reports

authors： Bouma MJ,van Iterson M,Janssen B,Mummery CL,Salvatori DCF,Freund C

更新日期：2017-05-09 00:00:00

abstract：:Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a m...

journal_title：Stem cell reports

authors： Avior Y,Lezmi E,Yanuka D,Benvenisty N

更新日期：2017-05-09 00:00:00

abstract：:The chromatin of naive embryonic stem cells (ESCs) has a largely open configuration, as evident by the lack of condensed heterochromatin and the hypomethylation of DNA. Several molecular mechanisms promoting this constellation were previously identified. Here we present evidence for an important epigenetic function of...

journal_title：Stem cell reports

authors： Rahjouei A,Pirouz M,Di Virgilio M,Kamin D,Kessel M

更新日期：2017-04-11 00:00:00

abstract：:Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure. Previous studies demonstrate the potential of liver organoids for modeling genetic diseases. To examine the poss...

journal_title：Stem cell reports

authors： Kruitwagen HS,Oosterhoff LA,Vernooij IGWH,Schrall IM,van Wolferen ME,Bannink F,Roesch C,van Uden L,Molenaar MR,Helms JB,Grinwis GCM,Verstegen MMA,van der Laan LJW,Huch M,Geijsen N,Vries RG,Clevers H,Rothuizen J,Schota

更新日期：2017-04-11 00:00:00

abstract：:Human stem cell models have the potential to provide platforms for phenotypic screens to identify candidate treatments and cellular pathways involved in the pathogenesis of neurodegenerative disorders. Amyloid precursor protein (APP) processing and the accumulation of APP-derived amyloid β (Aβ) peptides are key proces...

journal_title：Stem cell reports

authors： Brownjohn PW,Smith J,Portelius E,Serneels L,Kvartsberg H,De Strooper B,Blennow K,Zetterberg H,Livesey FJ

更新日期：2017-04-11 00:00:00

abstract：:Induced pluripotent stem cells were created from a pancreas agenesis patient with a mutation in GATA6. Using genome-editing technology, additional stem cell lines with mutations in both GATA6 alleles were generated and demonstrated a severe block in definitive endoderm induction, which could be rescued by re-expressio...

journal_title：Stem cell reports

authors： Tiyaboonchai A,Cardenas-Diaz FL,Ying L,Maguire JA,Sim X,Jobaliya C,Gagne AL,Kishore S,Stanescu DE,Hughes N,De Leon DD,French DL,Gadue P

更新日期：2017-03-14 00:00:00

abstract：:A method to maintain and rebuild ureteric bud (UB)-like structures from UB cells in vitro could provide a useful tool for kidney regeneration. We aimed in our present study to establish a serum-free culture system that enables the expansion of UB progenitor cells, i.e., UB tip cells, and reconstruction of UB-like stru...

journal_title：Stem cell reports

authors： Yuri S,Nishikawa M,Yanagawa N,Jo OD,Yanagawa N

更新日期：2017-02-14 00:00:00

abstract：:Maternal nicotine exposure causes alteration of gene expression and cardiovascular programming. The discovery of nicotine-medicated regulation in cardiogenesis is of major importance for the study of cardiac defects. The present study investigated the effect of nicotine on cardiac gene expression and epigenetic regula...

journal_title：Stem cell reports

authors： Jiang XY,Feng YL,Ye LT,Li XH,Feng J,Zhang MZ,Shelat HS,Wassler M,Li Y,Geng YJ,Yu XY

更新日期：2017-02-14 00:00:00

abstract：:Dynamic spatiotemporal modification of chromatin around DNA damage is vital for efficient DNA repair. Normal stem cells exhibit an attenuated DNA damage response (DDR), inefficient DNA repair, and high radiosensitivity. The impact of unique chromatin characteristics of stem cells in DDR regulation is not yet recognize...

journal_title：Stem cell reports

authors： Meyer B,Fabbrizi MR,Raj S,Zobel CL,Hallahan DE,Sharma GG

更新日期：2016-12-13 00:00:00

abstract：:We established two human embryonic stem cell (hESC) lines with a GGGGCC expansion in the C9orf72 gene (C9), and compared them with haploidentical and unrelated C9 induced pluripotent stem cells (iPSCs). We found a marked difference in C9 methylation between the cells. hESCs and parental fibroblasts are entirely unmeth...

journal_title：Stem cell reports

authors： Cohen-Hadad Y,Altarescu G,Eldar-Geva T,Levi-Lahad E,Zhang M,Rogaeva E,Gotkine M,Bartok O,Ashwal-Fluss R,Kadener S,Epsztejn-Litman S,Eiges R

更新日期：2016-11-08 00:00:00

abstract：:Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common changes in hPSCs are trisomies of chromosomes 1, 12, 17, and 20. Thus, hPSCs should be regularly screened for such aberration...

journal_title：Stem cell reports

authors： Baker D,Hirst AJ,Gokhale PJ,Juarez MA,Williams S,Wheeler M,Bean K,Allison TF,Moore HD,Andrews PW,Barbaric I

更新日期：2016-11-08 00:00:00

abstract：:Mesenchymal stem cells (MSCs) can donate mitochondria and rescue anthracycline-induced cardiomyocyte (CM) damage, although the underlying mechanisms remain elusive. We determined that the superior efficiency of mitochondrial transfer by human induced-pluripotent-stem-cell-derived MSCs (iPSC-MSCs) compared with bone ma...

journal_title：Stem cell reports

authors： Zhang Y,Yu Z,Jiang D,Liang X,Liao S,Zhang Z,Yue W,Li X,Chiu SM,Chai YH,Liang Y,Chow Y,Han S,Xu A,Tse HF,Lian Q

更新日期：2016-10-11 00:00:00

abstract：:Mitochondria are critical to neurogenesis, but the mechanisms of mitochondria in neurogenesis have not been well explored. We fully characterized mitochondrial alterations and function in relation to the development of human induced pluripotent stem cell (hiPSC)-derived dopaminergic (DA) neurons. Following directed di...

journal_title：Stem cell reports

authors： Fang D,Qing Y,Yan S,Chen D,Yan SS

更新日期：2016-10-11 00:00:00

abstract：:The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of muscle regeneration due to its high cell turnover. The resident satellite cells (the bona fide muscle stem cells) remain poorly characterized. Here we analyzed PC satellite cells with r...

journal_title：Stem cell reports

authors： Naldaiz-Gastesi N,Goicoechea M,Alonso-Martín S,Aiastui A,López-Mayorga M,García-Belda P,Lacalle J,San José C,Araúzo-Bravo MJ,Trouilh L,Anton-Leberre V,Herrero D,Matheu A,Bernad A,García-Verdugo JM,Carvajal JJ,Relaix F,L

更新日期：2016-09-13 00:00:00

abstract：:In human-to-mouse xenograft models, reconstitution of human hematopoiesis is usually B-lymphoid dominant. Here we show that the introduction of homozygous Kit(Wv) mutations into C57BL/6.Rag2(null)Il2rg(null) mice with NOD-Sirpa (BRGS) strongly promoted human multi-lineage reconstitution. After xenotransplantation of h...

journal_title：Stem cell reports

authors： Yurino A,Takenaka K,Yamauchi T,Nunomura T,Uehara Y,Jinnouchi F,Miyawaki K,Kikushige Y,Kato K,Miyamoto T,Iwasaki H,Kunisaki Y,Akashi K

更新日期：2016-09-13 00:00:00

abstract：:When comparing hepatic phenotypes between iPSC-derived hepatocyte-like cells from different liver disease patients, cell heterogeneity can confound interpretation. We proposed that homogeneous cell populations could be generated by fluorescence-activated cell sorting (FACS). Using cell-surface capture proteomics, we i...

journal_title：Stem cell reports

authors： Mallanna SK,Cayo MA,Twaroski K,Gundry RL,Duncan SA

更新日期：2016-09-13 00:00:00

abstract：:Tight regulation of the balance between self-renewal and differentiation of neural stem cells is crucial to assure proper neural development. In this context, Notch signaling is a well-known promoter of stemness. In contrast, the bifunctional brain-enriched microRNA miR-9/9(∗) has been implicated in promoting neuronal...

journal_title：Stem cell reports

authors： Roese-Koerner B,Stappert L,Berger T,Braun NC,Veltel M,Jungverdorben J,Evert BO,Peitz M,Borghese L,Brüstle O

更新日期：2016-08-09 00:00:00

abstract：:Meiosis is the key step in gametogenesis. However, the mechanism of mammalian meiosis remains poorly understood due to the lack of an in vitro model. Here, we report that retinoic acid (RA) is sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulat...

journal_title：Stem cell reports

authors： Wang S,Wang X,Ma L,Lin X,Zhang D,Li Z,Wu Y,Zheng C,Feng X,Liao S,Feng Y,Chen J,Hu X,Wang M,Han C

更新日期：2016-07-12 00:00:00

abstract：:The rigorous characterization of distinct induced pluripotent stem cells (iPSC) derived from multiple reprogramming technologies, somatic sources, and donors is required to understand potential sources of variability and downstream potential. To achieve this goal, the Progenitor Cell Biology Consortium performed compr...

journal_title：Stem cell reports

authors： Salomonis N,Dexheimer PJ,Omberg L,Schroll R,Bush S,Huo J,Schriml L,Ho Sui S,Keddache M,Mayhew C,Shanmukhappa SK,Wells J,Daily K,Hubler S,Wang Y,Zambidis E,Margolin A,Hide W,Hatzopoulos AK,Malik P,Cancelas JA,Aro

更新日期：2016-07-12 00:00:00

abstract：:Human pluripotent stem cells (hPSCs) offer a renewable source of cells that can be expanded indefinitely and differentiated into virtually any type of cell in the human body, including neurons. This opens up unprecedented possibilities to study neuronal cell and developmental biology and cellular pathology of the nerv...

journal_title：Stem cell reports

authors： Rigamonti A,Repetti GG,Sun C,Price FD,Reny DC,Rapino F,Weisinger K,Benkler C,Peterson QP,Davidow LS,Hansson EM,Rubin LL

更新日期：2016-06-14 00:00:00

abstract：:In this study we dissected retinal organoid morphogenesis in human embryonic stem cell (hESC)-derived cultures and established a convenient method for isolating large quantities of retinal organoids for modeling human retinal development and disease. Epithelialized cysts were generated via floating culture of clumps o...

journal_title：Stem cell reports

authors： Lowe A,Harris R,Bhansali P,Cvekl A,Liu W

更新日期：2016-05-10 00:00:00

abstract：:Heterozygous mutations in the glucocerebrosidase gene (GBA) represent the strongest common genetic risk factor for Parkinson's disease (PD), the second most common neurodegenerative disorder. However, the molecular mechanisms underlying this association are still poorly understood. Here, we have analyzed ten independe...

journal_title：Stem cell reports

authors： Fernandes HJ,Hartfield EM,Christian HC,Emmanoulidou E,Zheng Y,Booth H,Bogetofte H,Lang C,Ryan BJ,Sardi SP,Badger J,Vowles J,Evetts S,Tofaris GK,Vekrellis K,Talbot K,Hu MT,James W,Cowley SA,Wade-Martins R

更新日期：2016-03-08 00:00:00

abstract：:Stem cell-derived somatic cells represent an unlimited resource for basic and translational science. Although promising, there are significant hurdles that must be overcome. Our focus is on the generation of the major cell type of the human liver, the hepatocyte. Current protocols produce variable populations of hepat...

journal_title：Stem cell reports

authors： Cameron K,Tan R,Schmidt-Heck W,Campos G,Lyall MJ,Wang Y,Lucendo-Villarin B,Szkolnicka D,Bates N,Kimber SJ,Hengstler JG,Godoy P,Forbes SJ,Hay DC

更新日期：2015-12-08 00:00:00

abstract：:Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to id...

journal_title：Stem cell reports

authors： Jung P,Sommer C,Barriga FM,Buczacki SJ,Hernando-Momblona X,Sevillano M,Duran-Frigola M,Aloy P,Selbach M,Winton DJ,Batlle E

更新日期：2015-12-08 00:00:00

abstract：:Self-renewal potential and multipotency are hallmarks of a stem cell. It is generally accepted that acquisition of such stemness requires rejuvenation of somatic cells through reprogramming of their genetic and epigenetic status.We show here that a simple block of cell differentiation is sufficient to induce and maint...

journal_title：Stem cell reports

authors： Ikawa T,Masuda K,Huijskens MJAJ,Satoh R,Kakugawa K,Agata Y,Miyai T,Germeraad WTV,Katsura Y,Kawamoto H

更新日期：2015-11-10 00:00:00

abstract：:Activation of Wnt/β-catenin signaling can induce both self-renewal and differentiation in naive pluripotent embryonic stem cells (ESCs). To gain insights into the mechanism by which Wnt/β-catenin regulates ESC fate, we screened and characterized its downstream targets. Here, we show that the self-renewal-promoting eff...

journal_title：Stem cell reports

authors： Qiu D,Ye S,Ruiz B,Zhou X,Liu D,Zhang Q,Ying QL

更新日期：2015-09-08 00:00:00

abstract：:Primordial germ cells (PGCs) are fate restricted to differentiate into gametes in vivo. However, when removed from their embryonic niche, PGCs undergo reversion to pluripotent embryonic germ cells (EGCs) in vitro. One of the major differences between EGCs and embryonic stem cells (ESCs) is variable methylation at impr...

journal_title：Stem cell reports

authors： Oliveros-Etter M,Li Z,Nee K,Hosohama L,Hargan-Calvopina J,Lee SA,Joti P,Yu J,Clark AT

更新日期：2015-09-08 00:00:00

abstract：:The in vitro derivation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is complicated by the existence of multiple overlapping embryonic blood cell programs called primitive, erythromyeloid progenitor (EMP), and definitive. As HSCs are only generated during the definitive stage of hematopoiesis,...

journal_title：Stem cell reports

authors： Clarke RL,Robitaille AM,Moon RT,Keller G

更新日期：2015-08-11 00:00:00

abstract：:Primordial germ cells (PGCs) are lineage-restricted unipotent cells that can dedifferentiate into pluripotent embryonic germ cells (EGCs). Here we performed whole-transcriptome analysis during the conversion of PGCs into EGCs, a process by which cells acquire pluripotency. To examine the molecular mechanism underlying...

journal_title：Stem cell reports

authors： Nagamatsu G,Saito S,Takubo K,Suda T

更新日期：2015-07-14 00:00:00

abstract：:Teratoma formation is the gold standard assay for testing the capacity of human pluripotent stem cells to differentiate into all embryonic germ layers. Although widely used, little effort has been made to transform this qualitative assay into a quantitative one. Using gene expression data from a wide variety of cells,...

journal_title：Stem cell reports

authors： Avior Y,Biancotti JC,Benvenisty N

更新日期：2015-06-09 00:00:00

abstract：:Pro-fibrotic microenvironments of scars and tumors characterized by increased stiffness stimulate mesenchymal stromal cells (MSCs) to express α-smooth muscle actin (α-SMA). We investigated whether incorporation of α-SMA into contractile stress fibers regulates human MSC fate. Sorted α-SMA-positive MSCs exhibited high ...

journal_title：Stem cell reports

authors： Talele NP,Fradette J,Davies JE,Kapus A,Hinz B

更新日期：2015-06-09 00:00:00

abstract：:Reprogramming of somatic cells produces induced pluripotent stem cells (iPSCs) that are invaluable resources for biomedical research. Here, we extended the previous transcriptome studies by performing RNA-seq on cells defined by a combination of multiple cellular surface markers. We found that transcriptome changes du...

journal_title：Stem cell reports

authors： Tanaka Y,Hysolli E,Su J,Xiang Y,Kim KY,Zhong M,Li Y,Heydari K,Euskirchen G,Snyder MP,Pan X,Weissman SM,Park IH

更新日期：2015-06-09 00:00:00

abstract：:Research in human induced pluripotent stem cells (hiPSCs) is rapidly developing and there are expectations that this research may obviate the need to use human embryonic stem cells (hESCs), the ethics of which has been a subject of controversy for more than 15 years. In this study, we investigated approximately 3,400 ...

journal_title：Stem cell reports

authors： Kobold S,Guhr A,Kurtz A,Löser P

更新日期：2015-05-12 00:00:00

abstract：:The differentiation of pluripotent stem cells to hepatocytes is well established, yet current methods suffer from several drawbacks. These include a lack of definition and reproducibility, which in part stems from continued reliance on recombinant growth factors. This has remained a stumbling block for the translation...

journal_title：Stem cell reports

authors： Siller R,Greenhough S,Naumovska E,Sullivan GJ

更新日期：2015-05-12 00:00:00

abstract：:Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during...

journal_title：Stem cell reports

authors： Pasqualini FS,Sheehy SP,Agarwal A,Aratyn-Schaus Y,Parker KK

更新日期：2015-03-10 00:00:00

abstract：:Precise methods for transgene regulation are important to study signaling pathways and cell lineages in biological systems where gene function is often recycled within and across lineages. We engineered a genetic toolset for flexible transgene regulation in these diverse cellular contexts. Specifically, we created an ...

journal_title：Stem cell reports

authors： Akhtar AA,Molina J,Dutra-Clarke M,Kim GB,Levy R,Schreiber-Stainthorp W,Danielpour M,Breunig JJ

更新日期：2015-03-10 00:00:00

abstract：:In placental mammals, balanced expression of X-linked genes is accomplished by X chromosome inactivation (XCI) in female cells. In humans, random XCI is initiated early during embryonic development. To investigate whether reprogramming of female human fibroblasts into induced pluripotent stem cells (iPSCs) leads to re...

journal_title：Stem cell reports

authors： Barakat TS,Ghazvini M,de Hoon B,Li T,Eussen B,Douben H,van der Linden R,van der Stap N,Boter M,Laven JS,Galjaard RJ,Grootegoed JA,de Klein A,Gribnau J

更新日期：2015-02-10 00:00:00

abstract：:In this article, Scott describes how a new counseling profession could support clinical sites and patients enrolling in stem cell clinical trials. A possible model is proposed, along with a curriculum that would provide counselors with the tools to address challenges facing the clinical stem cell field. Finally, a can...

journal_title：Stem cell reports

authors： Scott CT

更新日期：2015-01-13 00:00:00

abstract：:As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholin...

journal_title：Stem cell reports

authors： Tzung KW,Goto R,Saju JM,Sreenivasan R,Saito T,Arai K,Yamaha E,Hossain MS,Calvert MEK,Orbán L

更新日期：2015-01-13 00:00:00

abstract：:Reprogramming of fibroblasts to induced pluripotent stem cells (iPSCs) entails a mesenchymal to epithelial transition (MET). While attempting to dissect the mechanism of MET during reprogramming, we observed that knockdown (KD) of the epithelial-to-mesenchymal transition (EMT) factor SNAI1 (SNAIL) paradoxically reduce...

journal_title：Stem cell reports

authors： Unternaehrer JJ,Zhao R,Kim K,Cesana M,Powers JT,Ratanasirintrawoot S,Onder T,Shibue T,Weinberg RA,Daley GQ

更新日期：2014-11-11 00:00:00